Hello community!

Allen Institute for Brain Science scientist ?Alexh? compiled some thoughts on puzzle 03: Mouse V1, and here they are:

The biggest difference between this cell and the last was the lack of false positives. There was a bit of stuff around the soma, but very little. More importantly, I did not see any false branches (maybe a couple of very short ones)!

The dendrites are approximately 95% captured. There is one dendritic branch that was missed because it is very faint. One dendritic tip was missed because it had an abrupt change of direction; abrupt changes of direction are challenging to catch. There was also some tracing inaccuracy occuring because the dendrites cross each other very closely. Here is a screenshot, gold standard in blue, Mozak community consensus in pink:

The end of one dendrite was improperly continued on the branch of another dendrite. This can occur when branches cross each other very closely. One way to minimize this happening is to take some time and scan back and forth in the single plane view until you feel super confident in moving on.

Around 75% of the axon was captured this time, but even so, it was often discontinuous. The only completely missed axon branches were near the soma, which is usually a zone of lower signal-to-noise. This makes it easier to miss faint axons.

Overall, I thought this was a very good representation of the cell!

Now it is time for us to tackle a new challenge. Go here to get started.